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ATCC corpse e1 corynebacterium pseudogenitalium atcc 33035 gl542874 17142
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A Time schedule of Braf CA activation and UVB irradiation. B Kaplan–Meier curve of melanoma-free survival in mice treated perinatally with 4OHTx or 4OHTx+UVB. Inset, a representative picture of a melanoma in an adult Braf CA mouse. C Graph showing the percentage of mice that developed melanoma according to treatment and gene dose. D Graph showing the mean time to melanoma development according to treatment and genotype. E Analysis of tumor multiplicity in irradiated Braf mutant mice according to gene dose. F IHC analysis of flank skin harvested from Tyr::Cre ERT2 ; Braf CA/+ ( B ) mice at various time points after the indicated treatments. The arrowheads indicate melanocytes (Red: in H&E staining, white: Ki67 or TYRP1 positive cells, yellow: <t>p16</t> positive cells); dashed lines delimitate the hair follicles; scale bars: black, 20 µm, white or red, 10 µm. G IHC and β-Gal staining in melanocytes isolated from B mice upon the indicated treatments. Right, semiquantitative analysis of IHC staining and β-Gal activity ( n = 3 experiments; 10 fields per condition).
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A Time schedule of Braf CA activation and UVB irradiation. B Kaplan–Meier curve of melanoma-free survival in mice treated perinatally with 4OHTx or 4OHTx+UVB. Inset, a representative picture of a melanoma in an adult Braf CA mouse. C Graph showing the percentage of mice that developed melanoma according to treatment and gene dose. D Graph showing the mean time to melanoma development according to treatment and genotype. E Analysis of tumor multiplicity in irradiated Braf mutant mice according to gene dose. F IHC analysis of flank skin harvested from Tyr::Cre ERT2 ; Braf CA/+ ( B ) mice at various time points after the indicated treatments. The arrowheads indicate melanocytes (Red: in H&E staining, white: Ki67 or TYRP1 positive cells, yellow: <t>p16</t> positive cells); dashed lines delimitate the hair follicles; scale bars: black, 20 µm, white or red, 10 µm. G IHC and β-Gal staining in melanocytes isolated from B mice upon the indicated treatments. Right, semiquantitative analysis of IHC staining and β-Gal activity ( n = 3 experiments; 10 fields per condition).
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A Time schedule of Braf CA activation and UVB irradiation. B Kaplan–Meier curve of melanoma-free survival in mice treated perinatally with 4OHTx or 4OHTx+UVB. Inset, a representative picture of a melanoma in an adult Braf CA mouse. C Graph showing the percentage of mice that developed melanoma according to treatment and gene dose. D Graph showing the mean time to melanoma development according to treatment and genotype. E Analysis of tumor multiplicity in irradiated Braf mutant mice according to gene dose. F IHC analysis of flank skin harvested from Tyr::Cre ERT2 ; Braf CA/+ ( B ) mice at various time points after the indicated treatments. The arrowheads indicate melanocytes (Red: in H&E staining, white: Ki67 or TYRP1 positive cells, yellow: <t>p16</t> positive cells); dashed lines delimitate the hair follicles; scale bars: black, 20 µm, white or red, 10 µm. G IHC and β-Gal staining in melanocytes isolated from B mice upon the indicated treatments. Right, semiquantitative analysis of IHC staining and β-Gal activity ( n = 3 experiments; 10 fields per condition).
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A Time schedule of Braf CA activation and UVB irradiation. B Kaplan–Meier curve of melanoma-free survival in mice treated perinatally with 4OHTx or 4OHTx+UVB. Inset, a representative picture of a melanoma in an adult Braf CA mouse. C Graph showing the percentage of mice that developed melanoma according to treatment and gene dose. D Graph showing the mean time to melanoma development according to treatment and genotype. E Analysis of tumor multiplicity in irradiated Braf mutant mice according to gene dose. F IHC analysis of flank skin harvested from Tyr::Cre ERT2 ; Braf CA/+ ( B ) mice at various time points after the indicated treatments. The arrowheads indicate melanocytes (Red: in H&E staining, white: Ki67 or TYRP1 positive cells, yellow: <t>p16</t> positive cells); dashed lines delimitate the hair follicles; scale bars: black, 20 µm, white or red, 10 µm. G IHC and β-Gal staining in melanocytes isolated from B mice upon the indicated treatments. Right, semiquantitative analysis of IHC staining and β-Gal activity ( n = 3 experiments; 10 fields per condition).
P16 Ma5 17142 Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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A Time schedule of Braf CA activation and UVB irradiation. B Kaplan–Meier curve of melanoma-free survival in mice treated perinatally with 4OHTx or 4OHTx+UVB. Inset, a representative picture of a melanoma in an adult Braf CA mouse. C Graph showing the percentage of mice that developed melanoma according to treatment and gene dose. D Graph showing the mean time to melanoma development according to treatment and genotype. E Analysis of tumor multiplicity in irradiated Braf mutant mice according to gene dose. F IHC analysis of flank skin harvested from Tyr::Cre ERT2 ; Braf CA/+ ( B ) mice at various time points after the indicated treatments. The arrowheads indicate melanocytes (Red: in H&E staining, white: Ki67 or TYRP1 positive cells, yellow: <t>p16</t> positive cells); dashed lines delimitate the hair follicles; scale bars: black, 20 µm, white or red, 10 µm. G IHC and β-Gal staining in melanocytes isolated from B mice upon the indicated treatments. Right, semiquantitative analysis of IHC staining and β-Gal activity ( n = 3 experiments; 10 fields per condition).
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A Time schedule of Braf CA activation and UVB irradiation. B Kaplan–Meier curve of melanoma-free survival in mice treated perinatally with 4OHTx or 4OHTx+UVB. Inset, a representative picture of a melanoma in an adult Braf CA mouse. C Graph showing the percentage of mice that developed melanoma according to treatment and gene dose. D Graph showing the mean time to melanoma development according to treatment and genotype. E Analysis of tumor multiplicity in irradiated Braf mutant mice according to gene dose. F IHC analysis of flank skin harvested from Tyr::Cre ERT2 ; Braf CA/+ ( B ) mice at various time points after the indicated treatments. The arrowheads indicate melanocytes (Red: in H&E staining, white: Ki67 or TYRP1 positive cells, yellow: <t>p16</t> positive cells); dashed lines delimitate the hair follicles; scale bars: black, 20 µm, white or red, 10 µm. G IHC and β-Gal staining in melanocytes isolated from B mice upon the indicated treatments. Right, semiquantitative analysis of IHC staining and β-Gal activity ( n = 3 experiments; 10 fields per condition).
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A Time schedule of Braf CA activation and UVB irradiation. B Kaplan–Meier curve of melanoma-free survival in mice treated perinatally with 4OHTx or 4OHTx+UVB. Inset, a representative picture of a melanoma in an adult Braf CA mouse. C Graph showing the percentage of mice that developed melanoma according to treatment and gene dose. D Graph showing the mean time to melanoma development according to treatment and genotype. E Analysis of tumor multiplicity in irradiated Braf mutant mice according to gene dose. F IHC analysis of flank skin harvested from Tyr::Cre ERT2 ; Braf CA/+ ( B ) mice at various time points after the indicated treatments. The arrowheads indicate melanocytes (Red: in H&E staining, white: Ki67 or TYRP1 positive cells, yellow: p16 positive cells); dashed lines delimitate the hair follicles; scale bars: black, 20 µm, white or red, 10 µm. G IHC and β-Gal staining in melanocytes isolated from B mice upon the indicated treatments. Right, semiquantitative analysis of IHC staining and β-Gal activity ( n = 3 experiments; 10 fields per condition).

Journal: Oncogene

Article Title: Transcriptional reprogramming triggered by neonatal UV radiation or Lkb1 loss prevents BRAF V600E -induced growth arrest in melanocytes

doi: 10.1038/s41388-025-03339-7

Figure Lengend Snippet: A Time schedule of Braf CA activation and UVB irradiation. B Kaplan–Meier curve of melanoma-free survival in mice treated perinatally with 4OHTx or 4OHTx+UVB. Inset, a representative picture of a melanoma in an adult Braf CA mouse. C Graph showing the percentage of mice that developed melanoma according to treatment and gene dose. D Graph showing the mean time to melanoma development according to treatment and genotype. E Analysis of tumor multiplicity in irradiated Braf mutant mice according to gene dose. F IHC analysis of flank skin harvested from Tyr::Cre ERT2 ; Braf CA/+ ( B ) mice at various time points after the indicated treatments. The arrowheads indicate melanocytes (Red: in H&E staining, white: Ki67 or TYRP1 positive cells, yellow: p16 positive cells); dashed lines delimitate the hair follicles; scale bars: black, 20 µm, white or red, 10 µm. G IHC and β-Gal staining in melanocytes isolated from B mice upon the indicated treatments. Right, semiquantitative analysis of IHC staining and β-Gal activity ( n = 3 experiments; 10 fields per condition).

Article Snippet: Primary antibodies against Melan-A (MA5-15237; IHC 1:100), KIF14 (PA5-87769; WB 1:1000; IHC 1:100), p16 Ink4a (MA5-17142; IHC 1:100), S100B (#710363; IHC 1:100), CHTF18 (A301-883A; IHC 1:50), BNC1 (PA5-85984; IHC 1:200) and p21 CIP1/WAF1 (MS-891-P1; WB 1:1000) were purchased from Thermo Scientific (Waltham, MA, USA).

Techniques: Activation Assay, Irradiation, Mutagenesis, Staining, Isolation, Immunohistochemistry, Activity Assay

A IHC analysis of flank skin from Braf CA/+ ; Lkb1 F/F mice harvested at various time points after the indicated treatments. The arrowheads indicate melanocytes. (Red: in H&E staining, white: Ki67 or TYRP1 positive cells, yellow: p16 positive cells); dashed lines delimitate the hair follicles; scale bars: black, 20 µm, white or red, 10 µm. B Analysis of the upregulated gene lists from the B;L vs. B and B + UVB vs. B melanocyte gene expression profiles. The graphs show the terms enriched in the common genes, colored according to the p values obtained from Metascape. Significant identified protein-protein networks of the upregulated genes (log 2 FC > 0.265) in B + UVB and B ; L melanocytes compared to melanocytes expressing only Braf V600E ( B ). C Analysis of the downregulated gene lists from the B;L vs. B and B + UVB vs. B melanocyte gene expression profiles. The graphs show the terms enriched in the common genes, colored according to the p values obtained from Metascape. Significant identified protein-protein networks of the downregulated genes (log 2 FC < −0.265) in B + UVB and B ; L melanocytes compared to melanocytes expressing only Braf V600E ( B ).

Journal: Oncogene

Article Title: Transcriptional reprogramming triggered by neonatal UV radiation or Lkb1 loss prevents BRAF V600E -induced growth arrest in melanocytes

doi: 10.1038/s41388-025-03339-7

Figure Lengend Snippet: A IHC analysis of flank skin from Braf CA/+ ; Lkb1 F/F mice harvested at various time points after the indicated treatments. The arrowheads indicate melanocytes. (Red: in H&E staining, white: Ki67 or TYRP1 positive cells, yellow: p16 positive cells); dashed lines delimitate the hair follicles; scale bars: black, 20 µm, white or red, 10 µm. B Analysis of the upregulated gene lists from the B;L vs. B and B + UVB vs. B melanocyte gene expression profiles. The graphs show the terms enriched in the common genes, colored according to the p values obtained from Metascape. Significant identified protein-protein networks of the upregulated genes (log 2 FC > 0.265) in B + UVB and B ; L melanocytes compared to melanocytes expressing only Braf V600E ( B ). C Analysis of the downregulated gene lists from the B;L vs. B and B + UVB vs. B melanocyte gene expression profiles. The graphs show the terms enriched in the common genes, colored according to the p values obtained from Metascape. Significant identified protein-protein networks of the downregulated genes (log 2 FC < −0.265) in B + UVB and B ; L melanocytes compared to melanocytes expressing only Braf V600E ( B ).

Article Snippet: Primary antibodies against Melan-A (MA5-15237; IHC 1:100), KIF14 (PA5-87769; WB 1:1000; IHC 1:100), p16 Ink4a (MA5-17142; IHC 1:100), S100B (#710363; IHC 1:100), CHTF18 (A301-883A; IHC 1:50), BNC1 (PA5-85984; IHC 1:200) and p21 CIP1/WAF1 (MS-891-P1; WB 1:1000) were purchased from Thermo Scientific (Waltham, MA, USA).

Techniques: Staining, Gene Expression, Expressing